General Methods: Lentivirus Production

Production of Transgenic Pigs Mediated by Pseudotyped Lentivirus and Sperm

Sperm-mediated gene transfer can be a very efficient method to produce transgenic pigs, however, the results from different laboratories had not been widely repeated. Genomic integration of transgene by injection of pseudotyped lentivirus to the perivitelline space has been proved to be a reliable route to generate transgenic animals. To test whether transgene in the lentivirus can be delivered...

General methods.

Fmoc-amino acids were obtained from GL Biochem (Shanghai, China). All the other Starting materials were obtained from Alfa. Chemical reagents and solvents were used as received from commercial sources.

Bayesian Methods: General Background ?

We note the main points of history, as a framework on which to hang many background remarks concerning the nature and motivation of Bayesian/Maximum Entropy methods. Experience has shown that these are needed in order to understand recent work and problems. A more complete account of the history, with many more details and references, is given in Jaynes (1978). The following discussion is essen...

Sequential second derivative general linear methods for stiff systems

‎Second derivative general linear methods (SGLMs) as an extension‎ ‎of general linear methods (GLMs) have been introduced to improve‎ ‎the stability and accuracy properties of GLMs‎. ‎The coefficients of‎ ‎SGLMs are given by six matrices‎, ‎instead of four matrices for‎ ‎GLMs‎, ‎which are obtained by solving nonlinear systems of order and‎ ‎usually Runge--Kutta stability conditions‎. ‎In this p...

Application of Iterative Methods for Solving General Riccati Equation